High-shear Mechanical Methods.
High-shear mechanical methods for cell disruption fall into four major classes: rotor-stator disruptors, valve-type processors, fixed-geometry processors and fixed orifice and constant pressure processors. (These fluid processing systems also are used extensively for homogenization and deaggregation of a wide range of materials – uses that will not be discussed here.) These processors all work by placing the bulk aqueous media under shear forces that literally pull the cells apart. These systems are especially useful for larger scale laboratory experiments (over 20 mL) and offer the option for large-scale production.
Read more about this topic: Cell Disruption
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