Usage
Regardless of the type of indicator used the imaging procedure is generally very similar. The spectral properties of the dye change upon binding to calcium. For example the dye could be fluorescent only after binding to calcium, or its excitation and emission spectra could be modified by the binding. Cells loaded with the indicator or expressing it in case of GECI, can be viewed using a fluorescence microscope, captured by a CCD camera and analyzed according to intensity, ultimately reflecting the Ca2+ status.
Read more about this topic: Calcium Imaging
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