Bolshoi (Russian: большой, meaning big, large, great) may refer to:
- Bolshoi Theatre, a major ballet and opera theatre in Moscow, Russia
- Bolshoi Ballet, resident ballet company at the Bolshoi Theatre
- The Bolshoi, an English post-punk band
- Bolshoi, an exceptionally large bell in Danilov Monastery, Moscow
- 26793 Bolshoi, a main-belt asteroid