Biota may refer to:
- Biota (ecology), the plant and animal life of a region
- Biota (taxonomy), a superdomain in taxonomy
- Biota (plant), an evergreen coniferous tree, Platycladus orientalis
- Biota (band), an avant-prog band from Colorado, USA
- A synonym for artificial life organizations
- Biota!, a planned aquarium operated by Zoological Society of London (ZSL) in east London.
- Biota, Zaragoza, a municipality in Zaragoza province, Spain
- Biota Holdings, an Australian biotech company