Bettini is a surname of Italian origin and it may refer to:
- Antonio Bettini (1396–1487), Italian clergyman and writer
- Domenico Bettini (born 1644), Italian painter of the Baroque era
- Gianni Bettini (1860–1938), Italian-American builder of phonographs
- Paolo Bettini (born 1974), Italian road-racing bicyclist
- Pietro Bettini (fl. 17th century), Italian engraver of the Baroque era
- Thomas Bettini former member of American rock back Jackyl
- Zelia Trebelli-Bettini (1838–1892), French opera singer
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