Bessie can be a surname, a nickname (diminutive), and a woman's given name. Bessie is an English diminutive of Elizabeth or Bessandra, and has been so used since the 16th century. Bessie is sometimes used as a name in its own right, rather than an informal diminutive.

Bessie may refer to:

  • A common or stereotypical name for cows
  • Bessie, a stuffed Beanie Baby cow toy
  • Bessie (lake monster), a lake monster in Lake Erie
  • Bessie Awards in dance, the New York Dance and Performance Awards
  • Bessie Smith, blues singer
  • Bessie Love, stage name of American film actress Juanita Horton
  • Alvah Bessie, screenwriter blacklisted as one of the Hollywood Ten
  • Diamond Bessie, murdered prostitute
  • Bessie Coleman, first African-American female aviator
  • Bessilyn Johnson, woman who inspired Scotty's Castle
  • A car used on the science fiction television series Doctor Who
  • Bessie, an asphalt-paver character in the movie Cars
  • Bessie Higgenbottom, titular character of the Nickelodeon TV channel cartoon, The Mighty B!