Common Gene Components
- oriS, repE - F
- for plasmid replication and regulation of copy number.
- parA and parB
- for partitioning F plasmid DNA to daughter cells during division and ensures stable maintenance of the BAC.
- A selectable marker
- for antibiotic resistance; some BACs also have lacZ at the cloning site for blue/white selection.
- T7 & Sp6
- phage promoters for transcription of inserted genes.
Read more about this topic: Bacterial Artificial Chromosome
Famous quotes containing the words common and/or components:
“This is really the common mentality of prisoners: they read with great attention all the articles that deal with illnesses and send away for treatises and “be your own doctor” or “emergency treatments” and end up by discovering that they have at least 300 or 400 illnesses, whose symptoms they are experiencing.”
—Antonio Gramsci (1891–1937)
“Hence, a generative grammar must be a system of rules that can iterate to generate an indefinitely large number of structures. This system of rules can be analyzed into the three major components of a generative grammar: the syntactic, phonological, and semantic components.”
—Noam Chomsky (b. 1928)