Anastasio may refer to:
People with the given name Anastasio:
- Anastasio Alfaro (1865-1951), Costa Rican zoologist, geologist and explorer
- Anastasio Aquino (1792-1833), Salvadoran indigenous leader
- Anastasio Ballestrero (1913-1998), Italian prelate of the Roman Catholic Church
- Anastasio Bustamante (1780-1853), President of Mexico
- Anastasio Somoza Debayle (1925-1980), President of Nicaragua
- Anastasio Somoza García (1896-1956), President of Nicaragua
- Anastasio Somoza Portocarrero (20th century), Nicaraguan anti-communist
People with the surname Anastasio:
- Anthony Anastasio (1906-1963), New York mobster and labor racketeer
- Kara Anastasio (21st century), American politician
- Michael R. Anastasio (born 1948), director of the Los Alamos National Laboratory
- Trey Anastasio (born 1964), American guitarist, composer, and vocalist