Advantages
Each base in this sequencing method is read twice. This changes the color of two adjacent color space calls, therefore in order to miscall a SNP, two adjacent colors must be miscalled. Because of this the SNP miscall rate is on the order of e^2, where e is the device error rate.
Read more about this topic: 2 Base Encoding
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“No advantages in this world are pure and unmixed.”
—David Hume (1711–1776)
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—Ralph Waldo Emerson (1803–1882)