Round 3
Draw was made live on BBC Radio Manchester on 17 February. All twenty Co-operative Championship teams were added into the competition in this round, as well as four teams from the French Elite One Championship, and winners of the 2008 Russian Championship, RC Lokomotiv Moscow. The matches were played on 7–8 March.
| Tie no | Home team | Score | Away team |
|---|---|---|---|
| 1 | Siddal | 6–10 | Swinton Lions |
| 2 | York City Knights | 50–10 | Wigan St Patricks |
| 3 | Featherstone Rovers | 94–2 | The Army |
| 41 | Queens | A–A | Doncaster |
| 5 | Pilkington Recs | 24–34 | Batley Bulldogs |
| 6 | Barrow Raiders | 44–12 | Blackpool Panthers |
| 7 | Gateshead Thunder | 42–38 | Whitehaven |
| 8 | Workington Town | 6–18 | Lézignan Sangliers |
| 9 | Leigh Centurions | 82–6 | RC Lokomotiv Moscow |
| 10 | Oldham Roughyeds | 26–8 | Sharlston |
| 11 | Sheffield Eagles | 22–6 | Toulouse Olympique |
| 12 | Leeds Met Carnegie | 24–38 | Rochdale Hornets |
| 13 | Widnes Vikings | 88–0 | Saddleworth |
| 14 | Wath Brow Hornets | 14–12 | London Skolars |
| 15 | Keighley Cougars | 30–24 | Pia Donkeys |
| 162 | Kells | 12–22 | Hunslet Hawks |
| 17 | Halifax | 80–16 | Loughborough University |
| 18 | Dewsbury Rams | 18–6 | AS Carcassonne |
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