Playoff
The two teams which won their groups with the least amount of available points went through to compete in a playoff for the remaining final tournament place. In the event of multiple teams sharing the same amount of points, the points margin and score between first and second places was taken into account. The two legs were played in both teams' home regions.
| Team 1 | Agg. | Team 2 | 1st leg | 2nd leg |
|---|---|---|---|---|
| Vojvodina | 3–0 | Midden | 2–0 | 1–0 |
First leg
| 11 April 2001 | ||
| Vojvodina | 2 – 0 | Midden |
Second leg
| 25 April 2001 | ||
| Midden | 0 – 1 | Vojvodina |
Read more about this topic: 2001 UEFA Regions' Cup