Transfection - RNA Transfection

RNA Transfection

RNA can also be transfected into cells to transiently express its coded protein, or to study RNA decay kinetics. The later application is referred as siRNA transfection or RNA silencing, and has become a major application in research (to replace the "knock-down" experiments, to study the expression of proteins, i.e. of Endothelin-1 ) with potential applications in gene-therapy.

A limitation of the silencing approach rely on the toxicity of the transfection for cells, and its suspected effect on the expression of other genes/proteins.