Splice may refer to:
- as connection of two or more pieces of linear material
- Rope splicing, joining two pieces of rope or cable by weaving the strands of each into the other
- Eye splice, a method of creating a permanent loop in the end of multi stranded rope by means of rope splicing.
- V-splice, joining two pieces of wood by mating a deep V in one to a long extension of the other
- Tape splice, the joining of audio tape
- Film Splice, the joining of film stock
- Electrical splice, the joining of wires in electrical wiring
- Optical splice, the joining of optical fibers:
- Fusion splicing, a permanent splice between two fibers
- Mechanical splice, a temporary splice between two fibers
- in genetics
- RNA splicing, a natural modification of ribonucleic acids
- Genetic engineering, also known as gene splicing, artificially joining pieces of genetic material
- Protein splicing, a natural process where inteins are removed
- in computer science
- splice (system call), a system call used to transfer data on Linux
- TCP splicing, postponement of the connection between the client and the server in computer networking
- An operation between two Linked lists, as in the programming language C++
- in mathematics
- Splicing rule, a transformation of formal languages
- Splicing, in mathematics is an operation for creating new knots from other knots (and links). The concept's name is inspired by and related to rope splicing.
- in other meanings
- Splice (film), a 2009 science fiction film
- Splicing veins, the connection of more than one vein to formulate a long vein conduit for vascular bypass surgery
- Splice, on a cricket bat, where the handle is joined to the body of the bat
- Splice the mainbrace, an order given aboard naval vessels to issue the crew with a drink
- Splice (ice cream), an Australian iced confectionery consisting of ice cream surrounded by fruit flavoured ice
- Spliced (TV series), a Canadian animated TV series by Teletoon and Nelvana
- Software release life cycle, or Software Product Life Cycle (SPLICE)