Principles
The basic principles of SNP array are the same as the DNA microarray. These are the convergence of DNA hybridization, fluorescence microscopy, and solid surface DNA capture. The three mandatory components of the SNP arrays are:
- The array that contains immobilized nucleic acid sequences of target.
- One or more labeled allele-specific oligonucleotide (ASO) probes.
- A detection system that records and interprets the hybridization signal.
To achieve relative concentration independence and minimal cross-hybridization, raw sequences and SNPs of multiple databases are scanned to design the probes. Each SNP on the array is interrogated with different probes. Depending on the purpose of experiments, the amount of SNPs present on an array is considered.
Read more about this topic: SNP Array
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