Advantages Over Previous Methods
In comparison to the previous over-coated plate-based methods, SPA has a number of advantages that makes it more popular:
- Assay flexibility - the concentration of the components in the assay can be adjusted to suit the user due to the higher surface area design of the SPA beads, hence providing the user flexibility in designing assay.
- Radioactive label reduction - the SPA beads allows a potential reduction in the quantity of radioactive labeling required due to its higher binding capacity, which gives a higher signal. This method also allows the user to optimize the sensitivity of the assay by altering the quantity of SPA beads.
- Convenient - the key component of the SPA assay, the beads, do not depend on a particular plate type or supplier, hence its wide availability.
- Bead assortment - there is a diversity of bead types to choose from to suit the need of the user and application.
- No separation step - allows binding measurement without separation step associated with earlier methods, which reduces errors and allows higher throughput.
Read more about this topic: Scintillation Proximity Assay
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