Sasebo or 佐世保 may refer to:
- Sasebo, Nagasaki, a city in Japan and a base of the Imperial Japanese Navy in the city, later of the Japan Maritime Self-Defense Force
- United States Fleet Activities Sasebo, a post-WWII United States Navy Base in the city
- Sasebo Line, a railway line in Japan
- Sasebo Station, a railway station in the city of Sasebo, Nagasaki Prefecture, in Japan
- Sasebo slashing, a murder which occurred in 2004