Gene Expression Assays
Reporter genes can also be used to assay for the expression of the gene of interest, which may produce a protein that has little obvious or immediate effect on the cell culture or organism. In these cases the reporter is directly attached to the gene of interest to create a gene fusion. The two genes are under the same promoter elements and are transcribed into a single messenger RNA molecule. The mRNA is then translated into protein. In these cases it is important that both proteins be able to properly fold into their active conformations and interact with their substrates despite being fused. In building the DNA construct, a segment of DNA coding for a flexible polypeptide linker region is usually included so that the reporter and the gene product will only minimally interfere with one another.
Read more about this topic: Reporter Gene
Famous quotes containing the words expression and/or assays:
“The psychological umbilical cord is more difficult to cut than the real one. We experience our children as extensions of ourselves, and we feel as though their behavior is an expression of something within us...instead of an expression of something in them. We see in our children our own reflection, and when we don’t like what we see, we feel angry at the reflection.”
—Elaine Heffner (20th century)
“Your bait of falsehood takes this carp of truth,
And thus do we of wisdom and of reach,
With windlasses and with assays of bias,
By indirections find directions out.”
—William Shakespeare (1564–1616)