A primer binding site is a region of a nucleotide sequence where an RNA or DNA single-stranded primer binds to start replication. The primer binding site is on one of the two complementary strands of a double-stranded nucleotide polymer, in the strand which is to be copied, or is within a single-stranded nucleotide polymer sequence.
A PCR primer binding site is a site where a polymerase chain reaction (PCR) primer binds, to prime duplication of a complement to an existing DNA or RNA sequence.
The HIV primer binding site is a structured RNA element in the genomes of retroviruses to which tRNA binds to initiate reverse transcription. In HIV, the tRNA is tRNA(3)(Lys) although it can use other tRNAs. It consists of 18 nucleotides and follows the U5 region of the 5'-long terminal repeat (LTR) of the retrovirus.
Famous quotes containing the words primer, binding and/or site:
“Your master Robin Hood lies dead,
Therefore sigh as you sing.
Here lie his primer and his beads,
His bent bow and his arrows keen,
His good sword and his holy cross:”
—Anthony Munday (1553–1633)
“With a binding like you’ve got, people are gonna want to know what’s in the book.”
—Alan Jay Lerner (1918–1986)
“It is not menstrual blood per se which disturbs the imagination—unstanchable as that red flood may be—but rather the albumen in the blood, the uterine shreds, placental jellyfish of the female sea. This is the chthonian matrix from which we rose. We have an evolutionary revulsion from slime, our site of biologic origins. Every month, it is woman’s fate to face the abyss of time and being, the abyss which is herself.”
—Camille Paglia (b. 1947)