Osuna is a town and municipality in the province of Seville, southern Spain, in the autonomous community of Andalusia. As of 2009, it has a population of c. 17,800.
Among famous people associated with Osuna is Juan de Ayala, the commander of the first European ship to enter the San Francisco Bay in California.
The battle of Munda, the last battle won by Julius Caesar in person, was probably fought outside of Osuna, halfway to Écija near La Lantejuela.
In Michelangelo Antonioni's film, The Passenger, the character of Locke (played by Jack Nicholson) is assassinated in a hotel located in Osuna (Hotel de la Gloria).