Life Support System (LSS)
The life support system (LSS) of the Frog Otolith Experiment Package (FOEP) maintains a regulated environment within the FOEP to assure the survival and normal functioning of the experimental specimens. The LSS is designed to meet the physiological requirements of two demotorized frogs weighing 350 g (12 oz) each. Frogs are demotorized by cutting the limb nerves, which reduces their metabolic rate. In this condition, the frogs require no artificial respiration and can remain healthy without being fed, for as long as a month. After being installed in the centrifuge the frogs are completely immersed in water, which serves as the medium for exchange of oxygen and carbon dioxide and heat through the frog's skin.
The LSS primarily consists of two closed loops: one containing liquid and the other containing gas. The lower bulkhead of the inner assembly structure provides mounting space for all LSS equipment. The oxygen supply system operates through these loops and includes a 4.5 cm³ capacity oxygen bottle, a pressure reducer and regulator, an artificial lung, CO2 absorber, and water supply. Limited control over the temperature of the frogs' environment is available by means of a water evaporator/heater.
Read more about this topic: Orbiting Frog Otolith
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