Mycroft may refer to:
- Family name
- Frank Mycroft (1873–1900), an English cricketer who played for Derbyshire between 1893 and 1895
- Thomas Mycroft (1848–1911), an English cricketer
- William Mycroft (1841–1894), an English cricketer
- Mycroft & Moran, an imprint of Arkham House publishers created in Sauk City, Wisconsin in 1945
- Given name
- Mycroft Holmes, Sherlock Holmes' brother in Arthur Conan Doyle's well-known detective novels
- The Confessions of Mycroft Holmes: a paper chase
- Other
- Ian Gillies, late question setter and arbiter of the BBC Radio 4 quiz series Brain of Britain
- Mycroft Holmes (computer), a self-aware computer in Robert A. Heinlein's 1966 novel The Moon Is a Harsh Mistress
- The Mycroft project, a collection of search plugins for web browsers
- Mycroft Next, a character from Jasper Fforde's Thursday Next series of books