The initialism MLF may refer to:
- Malolactic fermentation, a process in winemaking
- Maple Leaf Foods, a Canadian food processing company
- Mark Lewis-Francis (born 1982), a British athlete
- Mark Louie Fabon, a Philippine model
- Medial longitudinal fasciculus, fibres in the brain
- Micro-lead frame, a type of microchip package
- Mission laïque française, a French international non-profit education organization
- Multilateral Force, a 1960s proposed fleet of submarines and warships manned by NATO crews
- Mutant Liberation Front, a team of fictional terrorists/supervillains in the Marvel universe
- Microsoft Licensing Fulfillment
- Minister Louis Farrakhan (born 1933 as Louis Waldcott) a leader of the Nation of Islam