Mir-34 Micro RNA Precursor Family - Targets of MiR-34

Targets of MiR-34

Yamakuchi et al.. showed that miR-34a targets the silent information regulator 1 (SIRT1) gene:

"miR-34 inhibition of SIRT1 leads to an increase in acetylated p53 and expression of p21 and PUMA, transcriptional targets of p53 that regulate the cell cycle and apoptosis, respectively. Furthermore, miR-34 suppression of SIRT1 ultimately leads to apoptosis in WT human colon cancer cells but not in human colon cancer cells lacking p53. Finally, miR-34a itself is a transcriptional target of p53, suggesting a positive feedback loop between p53 and miR-34a. Thus, miR-34a functions as a tumor suppressor, in part, through a SIRT1-p53 pathway."

Recently Quantitative proteomics - SILAC approach was used to identify miR-34a targets at genome level in HEK293T cells.