Isolation of Pure Cultures
For single-celled eukaryotes, such as yeast, the isolation of pure cultures uses the same techniques as for bacterial cultures. Pure cultures of multicellular organisms are often more easily isolated by simply picking out a single individual to initiate a culture. This is a useful technique for pure culture of fungi, multicellular algae, and small metazoa, for example. m/M;
Developing pure culture techniques is crucial to the observation of the specimen in question. The most common method to isolate individual cells and produce a pure culture is to prepare a streak plate. The streak plate method is a way to physically separate the microbial population, and is done by spreading the inoculate back and forth with an inoculating loop over the solid agar plate. Upon incubation, colonies will arise and single cells will have been isolated from the biomass.
Read more about this topic: Microbiological Culture, Eukaryotic Cell Culture
Famous quotes containing the words isolation of, isolation, pure and/or cultures:
“The only happy talkers are dandies who extract pleasure from the very perishability of their material and who would not be able to tolerate the isolation of all other forms of composition; for most good talkers, when they have run down, are miserable; they know that they have betrayed themselves, that they have taken material which should have a life of its own, to dispense it in noises upon the air.”
—Cyril Connolly (1903–1974)
“Only connect! That was the whole of her sermon. Only connect the prose and the passion, and both will be exalted, and human love will be seen at its height. Live in fragments no longer. Only connect, and the beast and the monk, robbed of the isolation that is life to either, will die.”
—E.M. (Edward Morgan)
“What if all ponds were shallow? Would it not react on the minds of men? I am thankful that this pond was made deep and pure for a symbol. While men believe in the infinite some ponds will be thought to be bottomless.”
—Henry David Thoreau (1817–1862)
“There has never been in history another such culture as the Western civilization M a culture which has practiced the belief that the physical and social environment of man is subject to rational manipulation and that history is subject to the will and action of man; whereas central to the traditional cultures of the rivals of Western civilization, those of Africa and Asia, is a belief that it is environment that dominates man.”
—Ishmael Reed (b. 1938)