Isolation of Pure Cultures
For single-celled eukaryotes, such as yeast, the isolation of pure cultures uses the same techniques as for bacterial cultures. Pure cultures of multicellular organisms are often more easily isolated by simply picking out a single individual to initiate a culture. This is a useful technique for pure culture of fungi, multicellular algae, and small metazoa, for example. m/M;
Developing pure culture techniques is crucial to the observation of the specimen in question. The most common method to isolate individual cells and produce a pure culture is to prepare a streak plate. The streak plate method is a way to physically separate the microbial population, and is done by spreading the inoculate back and forth with an inoculating loop over the solid agar plate. Upon incubation, colonies will arise and single cells will have been isolated from the biomass.
Read more about this topic: Microbiological Culture, Eukaryotic Cell Culture
Famous quotes containing the words isolation of, isolation, pure and/or cultures:
“One of the most significant effects of age-segregation in our society has been the isolation of children from the world of work. Whereas in the past children not only saw what their parents did for a living but even shared substantially in the task, many children nowadays have only a vague notion of the nature of the parent’s job, and have had little or no opportunity to observe the parent, or for that matter any other adult, when he is fully engaged in his work.”
—Urie Bronfenbrenner (b. 1917)
“This [new] period of parenting is an intense one. Never will we know such responsibility, such productive and hard work, such potential for isolation in the caretaking role and such intimacy and close involvement in the growth and development of another human being.”
—Joan Sheingold Ditzion and Dennie Palmer (20th century)
“We are in fact convinced that if we are ever to have pure knowledge of anything, we must get rid of the body and contemplate things by themselves with the soul by itself. It seems, to judge from the argument, that the wisdom which we desire and upon which we profess to have set our hearts will be attainable only when we are dead and not in our lifetime.”
—Socrates (469–399 B.C.)
“A two-week-old infant cries an average of one and a half hours every day. This increases to approximately three hours per day when the child is about six weeks old. By the time children are twelve weeks old, their daily crying has decreased dramatically and averages less than one hour. This same basic pattern of crying is present among children from a wide range of cultures throughout the world. It appears to be wired into the nervous system of our species.”
—Lawrence Kutner (20th century)