A microbiological culture, or microbial culture, is a method of multiplying microbial organisms by letting them reproduce in predetermined culture media under controlled laboratory conditions. Microbial cultures are used to determine the type of organism, its abundance in the sample being tested, or both. It is one of the primary diagnostic methods of microbiology and used as a tool to determine the cause of infectious disease by letting the agent multiply in a predetermined medium. For example, a throat culture is taken by scraping the lining of tissue in the back of the throat and blotting the sample into a medium to be able to screen for harmful microorganisms, such as Streptococcus pyogenes, the causative agent of strep throat. Furthermore, the term culture is more generally used informally to refer to "selectively growing" a specific kind of microorganism in the lab.
Microbial cultures are foundational and basic diagnostic methods used extensively as a research tool in molecular biology. It is often essential to isolate a pure culture of microorganisms. A pure (or axenic) culture is a population of cells or multicellular organisms growing in the absence of other species or types. A pure culture may originate from a single cell or single organism, in which case the cells are genetic clones of one another.
For the purpose of gelling the microbial culture, the medium of agarose gel (agar) is used. Agar is a gelatinous substance derived from seaweed. A cheap substitute for agar is guar gum, which can be used for the isolation and maintenance of thermophiles.
Other articles related to "microbiological culture, cultures, culture":
... For single-celled eukaryotes, such as yeast, the isolation of pure cultures uses the same techniques as for bacterial cultures ... Pure cultures of multicellular organisms are often more easily isolated by simply picking out a single individual to initiate a culture ... This is a useful technique for pure culture of fungi, multicellular algae, and small metazoa, for example ...
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“Sanity consists in not being subdued by your means. Fancy prices are paid for position, and for the culture of talent, but to the grand interests, superficial success is of no account.”
—Ralph Waldo Emerson (18031882)