Study
Due to macromolecular crowding enzyme assays and biophysical measurements performed in dilute solution may fail to reflect the actual process and its kinetics taking place in the cytosol. One approach to produce more accurate measurements would be to use highly-concentrated extracts of cells, to try to maintain the cell contents in a more natural state. However, when using such extracts it is very difficult to study one process in isolation. Consequently, the crowded effects may be mimicked in vitro by adding high concentrations of an inert molecule such as polyethylene glycol or ficoll to assays containing purified components. However, using such artificial crowding agents can be complicated, as these crowding molecules can sometimes interact in other ways with the process being examined, such as by binding weakly to one of the components.
Read more about this topic: Macromolecular Crowding
Famous quotes containing the word study:
“A creative writer must study carefully the works of his rivals, including the Almighty. He must possess the inborn capacity not only of recombining but of re-creating the given world. In order to do this adequately, avoiding duplication of labor, the artist should know the given world.”
—Vladimir Nabokov (1899–1977)
“What is more subtle than this which ties me to the woman or man
that looks in my face?
Which fuses me into you now, and pours my meaning into you?
We understand men do we not?
What I promis’d without mentioning it, have you not accepted?
What the study could not teach—what the preaching could
not accomplish is accomplish’d, is it not?”
—Walt Whitman (1819–1892)
“If we wish to know the force of human genius, we should read Shakespeare. If we wish to see the insignificance of human learning, we may study his commentators.”
—William Hazlitt (1778–1830)