Lupo is an Italian word meaning wolf. It may refer to:
- Persons
- Lupo I of Aquitaine
- Lupo II of Gascony
- Lupo III Centule of Gascony
- Lupo (surname), persons having the surname Lupo
- Lupo family, a family of musicians at the English court in the 16th and 17th centuries
- The Italian nickname of Bulgaria-born volleyball player Lyubomir Ganev
- Industry
- Volkswagen Lupo, a small city car manufactured by Volkswagen
- Lupo-class Italian frigate
- Lupo SA, a major Brazilian manufacturer of socks, underwear, lingerie and sports apparel
- Fictional characters
- A character in the Rat-Man (comics)
- A character in the German Fix and Foxi comics
- Lupo (comics), a member of the Savage Land Mutates in the X-Men comics
- Cyrus Lupo, lead character in the long-running NBC legal drama, Law & Order.
- Jo Lupo, a supporting character in the SyFy Channel science-fiction program Eureka.
- Lupo the Butcher, the main character of the two-minute film of the same name.
- Film
- Lupo! Israeli film directed by Menahem Golan
- Other
- Lupo (dog) a dog belonging to Catherine, Duchess of Cambridge