JTS may mean:
- JTS Corporation, a defunct manufacturer of inexpensive IDE hard drives
- JTS engine, a gasoline direct injection engine produced by Alfa Romeo
- JTS Topology Suite, an open-source Java software library for spatial computing
- Java transaction service, a Java software library that implements the JTA Transaction Manager
- Jewish Theological Seminary of America, academic and spiritual centers of conservative Judaism
- Jimmy Two-Shoes, a Canadian animated series
- Johnny Two Shoes, a British gaming site
- The Journal of Theological Studies, a UK-based academic journal