Technique
According to SynapseWeb, this is the recipe for Golgi's staining technique:
- Immerse a block (approx. 10x5 mm) of formol-fixed (or paraformaldehyde- glutaraldehyde-perfused) brain tissue into a 2% aqueous solution of potassium dichromate for 2 days
- Dry the block shortly with filter paper.
- Immerse the block into a 2% aqueous solution of silver nitrate for another 2 days.
- Cut sections approx. 20-100 µm thick.
- Dehydrate quickly in ethanol, clear and mount (e.g., into Depex or Enthalan).
This technique has since been refined to substitute the silver precipitate with gold by immersing the sample in gold chloride then oxalic acid, followed by removal of the silver by sodium thiosulphate. This preserves a greater degree of fine structure with the ultrastructural details marked by small particles of gold.
Read more about this topic: Golgi's Method
Famous quotes containing the word technique:
“I cannot think that espionage can be recommended as a technique for building an impressive civilisation. It’s a lout’s game.”
—Rebecca West (1892–1983)
“The mere mechanical technique of acting can be taught, but the spirit that is to give life to lifeless forms must be born in a man. No dramatic college can teach its pupils to think or to feel. It is Nature who makes our artists for us, though it may be Art who taught them their right mode of expression.”
—Oscar Wilde (1854–1900)
“The audience is the most revered member of the theater. Without an audience there is no theater. Every technique learned by the actor, every curtain, every flat on the stage, every careful analysis by the director, every coordinated scene, is for the enjoyment of the audience. They are our guests, our evaluators, and the last spoke in the wheel which can then begin to roll. They make the performance meaningful.”
—Viola Spolin (b. 1911)