Laboratory Diagnosis
In Acanthamoeba infections, the diagnosis can be made from microscopic examination of stained smears of biopsy specimens (brain tissue, skin, cornea) or of corneal scrapings, which may detect trophozoites and cysts. Cultivation of the causal organism, and its identification by direct immunofluorescent antibody, may also prove useful. Laboratory workers and physicians often mistake the organisms on wet mount for monocytes and a diagnosis of viral meningitis is mistakenly given if the organisms are not motile. Heating a copper penny with an alcohol lamp and placing it on the wet mount slide will activate sluggish trophozoites and more rapidly make the diagnosis. If the person performing the spinal tap rapidly looks at the heated wet mount slide the trophozoites can be seen to swarm while monocytes do not.
Read more about this topic: Free-living Amoebozoa Infection
Famous quotes containing the word laboratory:
“The best work of artists in any age is the work of innocence liberated by technical knowledge. The laboratory experiments that led to the theory of pure color equipped the impressionists to paint nature as if it had only just been created.”
—Nancy Hale (b. 1908)