In biochemistry, equilibrium unfolding is the process of unfolding a protein or RNA molecule by gradually changing its environment, such as by changing the temperature or pressure, adding chemical denaturants, or applying force as with an atomic force microscope tip. Since equilibrium is maintained at all steps, the process is reversible (equilibrium folding). Equilibrium unfolding is used to determine the conformational stability of the molecule.
Read more about Equilibrium Unfolding: Theoretical Background, Chemical Denaturation, Thermal Denaturation, Other Forms of Denaturation
Famous quotes containing the words equilibrium and/or unfolding:
“There is a relation between the hours of our life and the centuries of time. As the air I breathe is drawn from the great repositories of nature, as the light on my book is yielded by a star a hundred millions of miles distant, as the poise of my body depends on the equilibrium of centrifugal and centripetal forces, so the hours should be instructed by the ages and the ages explained by the hours.”
—Ralph Waldo Emerson (1803–1882)
“It is the child in man that is the source of his uniqueness and creativeness, and the playground is the optimal milieu for the unfolding of his capacities and talents.”
—Eric Hoffer (1902–1983)