Culturing
Short-term culturing of E. bieneusi was achieved by inoculating duodenal aspirate and biopsy specimens into E6 and HLF monolayers. The short-term cultures lasted up to 6 months. After several weeks of culture, gram-positive spore-like structures measuring 1 to 1.2 um long were observed. Mature spores and sporoblasts with double rows of polar tubule coils were seen (Visvesvara 2002). Long term culturing seems to be unsuccessful.
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