Limitations
Because the Edman degradation proceeds from the N-terminus of the protein, it will not work if the N-terminal amino acid has been chemically modified or if it is concealed within the body of the protein. It also requires the use of either guesswork or a separate procedure to determine the positions of disulfide bridges, and concentrations of 1 picomole of peptide or above for discernible results.
Read more about this topic: Edman Degradation
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