DBI may refer to:
- dBi, decibel isotropic
- DBi Technologies Inc., a provider of text mining, scheduling and UI design component software, services and computer programs
- Beck Depression Inventory, a psychological test used to measure depression levels
- Davies–Bouldin index (DBI)
- Diazepam binding inhibitor
- Perl DBI, a database interface for Perl
- Phenformin, by the trade name DBI