Cruikshank is a surname and is a spelling variation of Cruickshank. Notable people with the surname include:
- Brad Cruikshank (born 1979), Canadian ice hockey winger
- Dan Cruikshank (born 1949) architectural historian and broadcaster
- George Cruikshank (1792–1878), English caricaturist
- Holly Cruikshank (born 1973), American dancer
- Isaac Cruikshank (1756–1811), Scottish painter and caricaturist
- Isaac Robert Cruikshank (1789–1856), caricaturist, illustrator, and portrait miniaturist
- Lucas Cruikshank (born 1993), web producer and creator of Fred, a web-based show on YouTube
- Marcus Henderson Cruikshank (1826–1881), Confederate States of America politician
- Sally Cruikshank (born 1949), American cartoonist and animator
- Thomas H. Cruikshank, former chairman and CEO of Halliburton Energy Services
- William Cruikshank (disambiguation), several people