Controversy
Originally proposed by Roger Klein, the Columnmaster 4000 quickly became the subject of a hot debate surrounding its efficacy. While, in principle, the column would be an effective tool in partially automating a tedious purification procedure, the device failed to perform adequately during testing. Klein's support for the project was met with great opposition by his laboratory supervisor, Chris Nau. Nau pointed out that not only did the use of the column require more work during its set-up, running, and break-down than running chitin columns by hand, but also increased the chance of error during purification. Possible exposure to contaminants, resulting from glassware and rubber hosing, was also cited as a disadvantage of this technique.
The Columnmaster 4000 has yet to be successfully used in the application of column chromatography.
Read more about this topic: Columnmaster 4000
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