Benacerraf (Hebrew: בנאסרף Ben-Aseraf) is a surname. Notable people with the surname include:
- Baruj Benacerraf (Hebrew: ברוך בנאסרף Bārūkh Ben-Aseraf; born 1920), Venezuelan immunologist of Jewish Moroccan and Algerian origin; elder brother of Paul
- Margot Benacerraf (born 1926), Venezuelan film director
- Paul Benacerraf (born 1931), French-American philosopher; younger brother of Baruj
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