Assiniboine may refer to:
- People
- Assiniboine people, a Siouan Native American/First Nations people originally from the Northern Great Plains of the United States and Canada
- Assiniboine language, one of the Siouan languages
- Places
- Assiniboine River, a river that runs through the prairies of Western Canada in Saskatchewan and Manitoba
- Mount Assiniboine in eastern British Columbia, Canada
- Fort Assiniboine, Alberta, Canada
- Fort Assinniboine, Montana, U.S.
- Winnipeg—Assiniboine, a former Canadian federal electoral district
- Ships
- HMCS Assiniboine (I18), River-class destroyer, served 1939-1945
- HMCS Assiniboine (DDH 234), St. Laurent-class destroyer, served 1956-1988
- Other
- Assiniboine Herald, one of the heralds at the Canadian Heraldic Authority