Ano or ANO may refer to:
- Ano, Ethiopia
- Ano family, a noble family of Japan
- Ano (sport), a sport with two 12 cm balls, conducted at Tuvalu
- Abu Nidal Organization, Palestinian Revolutionary Council, founded by Abu Nidal
- Air Navigation Order, the laws of the air
- Akhurian River, also Ano Jur
- Alliance of the New Citizen, a political party of Slovakia
- Anoctamin, a group of proteins, e.g. anoctamin 1
- Arkansas Nuclear One, a nuclear power plant near Russellville, Arkansas
- An abbreviation of A. N. Other, a placeholder name or pseudonym used by a person wishing to be anonymous