Agnello is a surname. People with this surname include:
- John Agnello, American music producer
- Carmine Agnello (born 1960), New York mobster
- Chris Agnello (born 1968), American soccer coach
- Carmine Gotti Agnello (born 1986)
- John Gotti Agnello (born 1987)
- Frank Gotti Agnello (born 1990)