Scientific Work
Pfeffer was a pioneer of modern plant physiology. His scientific interests included the thermonastic and photonastic movements of flowers, the nyctinastic movements of leaves, protoplastic physics and photosynthesis. In 1877, while researching plant metabolism, Pfeffer developed a semi-porous membrane to study the phenomena of osmosis. The eponymous "Pfeffer cell" is named for the osmometric device he constructed for determining the osmotic pressure of a solution.
During his tenure at Leipzig, Pfeffer published an article on the use of photography to study plant growth. He wanted to extend the chronophotographic experiments of Étienne-Jules Marey (1830-1904) by producing a short film involving the stages of plant growth. This "movie" would be filmed over a period of weeks by frame-at-a-time exposure taken at regular spaced intervals. Later, time-lapse photography would become a commonplace procedure.
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“Anyone who is practically acquainted with scientific work is aware that those who refuse to go beyond fact rarely get as far as fact; and anyone who has studied the history of science knows that almost every great step therein has been made by the anticipation of Nature.”
—Thomas Henry Huxley (182595)