Prokaryotic Ubiquitin-like Protein (Pup)
Recently, functional analog of ubiquitin had been found in prokaryote. Prokaryotic ubiquitin-like protein (Pup) serves the same function (targeting proteins for degradations), although the enzymology of ubiquitylation and pupylation is different. In contrast to the three-step reaction of ubiquitylation, pupylation requires two steps, therefore only two enzymes are involved in pupylation.
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