Tsubame can refer to:
- Tsubame, Niigata, a city in Japan
- Tsubame (train), the name of a Japanese train service
- Tsubame Ozuno, a character in the anime and manga series Urusei Yatsura
- Sanjō Tsubame, a character in the anime and manga series Rurouni Kenshin
- Tsubame, an album by Merzbow
- Tsubame (supercomputer), a supercomputer in Japan