TSU
- Taiwan Solidarity Union
- Tarleton State University
- Tartu State University (former name of University of Tartu)
- Tbilisi State University
- Texas Southern University
- Texas State University–San Marcos (The abbv. TSU is discouraged by the university)
- Tennessee State University
- The Sunshine Underground, an indie rock band from Leeds, England
- Time Sharing User in mainframes
- National Research Tomsk State University
- Toy Soldiers Unite, official fan club of Dr. Steel, a steampunk industrial band.
- Trade Services Utility, a banking initiative for improving the Financial Supply Chain
- Trans-Species Unlimited, a national animal rights group, co-founded by Giorgio P. Cave and Dana Stuchell
- Trinity Southwest University
- Tri-State University
- Truman State University
- Tula State University
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