Other
- "TKO" (Babylon 5), an episode of Babylon 5
- TKO, a 2007 film starring Dianna Agron
- The Kindness Offensive, a North London group known for random acts of kindness
- Taylor KO Factor, a measurement of the stopping power of a firearms cartridge
- TKO Software, a defunct software company
- Tseung Kwan O, a new town in Hong Kong
- MTR station code for Tseung Kwan O Station
- Triple knockout, in molecular biology
- "To keep open", a medical acronym for an intravenous drip that is flowing just enough to keep the IV open for future use
- Three Kingdoms Online, a browser based strategy game
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