- David Bowie – Performance: "America", "Heroes"
- Billy Crystal – Comedy Routine
- Tom Daschle – Speech
- Bon Jovi – Performance: "Livin' on a Prayer", "Wanted Dead Or Alive", "It's My Life"
- Mark Wahlberg – Jay-Z's Introduction
- Jay-Z – Performance: "Izzo (H.O.V.A.)"
- Goo Goo Dolls – Performance: "American Girl", "Iris"
- Susan Sarandon – Speech
- Leonardo DiCaprio and Robert De Niro – Introduce Martin Scorsese short film
- Martin Scorsese – Short film: "The Neighborhood"
- Billy Joel – Performance: "Miami 2017 (Seen the Lights Go Out on Broadway)", "New York State of Mind"
- Will Ferrell – (as President George W. Bush) – Comedy Routine: On The Taliban
- Chris Kattan – Introduce Destiny's Child
- Destiny's Child- Performance: "Emotion", "Walk With Me (Gospel Medley)"
- Harrison Ford – Thank you to Sponsors, Introduce Eric Clapton
- Eric Clapton and Buddy Guy – Performance: "Hoochie Coochie Man"
- Christy Turlington – Introduce Edward Burns short film
- Edward Burns – Short film: "Lovely Day"
- James Lipton – Introduce Operaman
- Adam Sandler – (as Operaman) – Sings Operaman
- Meg Ryan – Introduce Backstreet Boys
- Backstreet Boys – Performance: "Quit Playing Games (With My Heart)"
- David Spade – Introduce Melissa Etheridge
- Melissa Etheridge – Performance: "Come To My Window", "Born To Run"
- Halle Berry – Introduce Spike Lee short film
- Spike Lee – Short film: "Come Rain or Come Shine"
- John Cusack – Introduce The Who
- The Who – Performance: "Who Are You", "Baba O'Riley", "Behind Blue Eyes" "Won't Get Fooled Again"
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- George Pataki – Speech
- John Cusack – Introduce Woody Allen short film
- Woody Allen – Short film: "Sounds from the Town I Love"
- Mike Myers – Introduce Mick Jagger and Keith Richards
- Mick Jagger and Keith Richards – Performance: "Salt of the Earth", "Miss You"
- Howard Stern – Introduce short film: "I Am A New Yorker"
- Julia Styles – Introduce Macy Gray
- Macy Gray – Performance: "With A Little Help From My Friends"
- Hillary Clinton – Introduce Jerry Seinfeld short film
- Jerry Seinfeld – Short film: "The Greatest City on Earth"
- Bill Clinton – Introduce James Taylor
- James Taylor – Performance: "Fire and Rain", "Up on the Roof"
- Michael J. Fox- Introduce Rudy Giuliani
- Rudy Giuliani – Speech
- Jimmy Fallon – 80's Hits to "You Can't Touch This"
- Jon Bon Jovi – Introduce Kevin Smith short film
- Kevin Smith – Short film: "Why I Love #$%&@ New York"
- John Mellencamp – Performance: "Peaceful World"
- John Mellencamp and Kid Rock – Performance: "Pink Houses"
- Hilary Swank – Introduce Five For Fighting
- Five For Fighting – Performance: "Superman (It's Not Easy)"
- Janet Jackson – Performance: "Together Again"
- Elton John – Performance: "I Want Love", "Mona Lisas and Mad Hatters"
- Elton John and Billy Joel – Performance: "Your Song"
- Jim Carrey – Introduce Paul McCartney
- Paul McCartney – Performance: "I'm Down", "Lonely Road", "From a Lover to a Friend", "Yesterday", "Freedom" (new song), "Let It Be" and "Freedom" (reprise)
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