In 1995, Burns auditioned for Blue's Clues, thinking it was another voice-over role. He showed up with long hair and an earring; "I was a bit of a skate rat", he said. Initially, the Nickelodeon executives were not supportive of Burns hosting their new show; in subsequent auditions the show's creators requested that he dress more conservatively. (Burns reported that the creators, in a call-back phone conversation, asked him, "Could you not look like you tomorrow morning?") It became apparent, however, that he was the favorite with preschool test audiences. Executive producer and co-creator Traci Paige Johnson reported that of the 100 people they auditioned, Burns was "the realest". As Dr. Alice Wilder, Nickelodeon's Director of Research and Development said, "here was just something about this kid, who was fresh out of Pennsylvania, who just knew where to look in the camera to really talk to kids. He was just right."
From its premiere, Blue's Clues was an instant hit, due to Steve Burns' performances as much as the show's format. He became "a superstar" among his audience and their parents, but unknown to everyone else, and enjoyed what he called being a "micro-celebrity, about as small a celebrity as you can be." As the New York Times reported, he "developed an avid following among both preteen girls and mothers. The former send torrents of e-mail; the latter scrutinize the show with an intensity that might make even Elmo, the red Muppet, blush." In 2000, People magazine included Burns in their annual list of America's most eligible bachelors. According to writer Diane Tracy, Burns was "destined for the part". Also according to Tracy, Burns was not the typical children's television host: "There is nothing syrupy about him—his humor is sometimes borderline offbeat, but never inappropriate for preschoolers".
Burns became "very involved" with the production of Blue's Clues from the beginning. One of the most challenging aspects of being the host of the show was performing in front of the "blue screen" before the animation was added. Burns called it "maddening" and likened it to "acting at the bottom of a swimming pool."
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