Method
To quantify the activity of steapsin in vitro, olive oil was used as the fat (triglycerides) to be broken down. Steapsin was added and various experiments were conducted which thus digested olive oil to form glycerol and free fatty acids. The free fatty acid formation caused the mixture to turn acidic, thus phenolphthalein indicator was added and the mixture was titrated against 0.1M NaOH. Therefore, the steapsin activity correlates to the amount of NaOH added, and subsequent experiments can be quantified and compared this way. To simulate the factors of the stomach, all experiments were carried out at 40 C.
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