Example
The Bradford assay is a colorimetric assay that measures protein concentration. The reagent Coomassie Brilliant Blue turns blue when it binds to arginine and aromatic amino acids present in proteins, thus increasing the absorbance of the sample. The absorbance is measured using a spectrophotometer, at the maximum absorbance frequency (Amax)of the blue dye (which is 595 nm). In this case, the greater the absorbance, the higher the protein concentration.
Data for known concentrations of protein are used to make the standard curve, plotting concentration on the X axis, and the assay measurement on the Y axis. The same assay is then performed with samples of unknown concentration. To analyze the data, one locates the measurement on the Y-axis that corresponds to the assay measurement of the unknown substance and follows a line to intersect the standard curve. The corresponding value on the X-axis is the concentration of substance in the unknown sample.
Read more about this topic: Standard Curve
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