Identification
S. marcescens is a motile organism and can grow in temperatures ranging from 5–40°C and in pH levels ranging from 5 to 9. It is differentiated from other Gram-negative bacteria by its ability to perform casein hydrolysis, which allows it to produce extracellular metalloproteinases which are believed to function in cell-to-extracellular matrix interactions. S. marcescens also exhibits tryptophan and citrate degradation. One of the end products of tryptophan degradation is pyruvic acid, which is then incorporated into different metabolic processes of S. marcescens. A final product of citrate degradation is carbon. Thus, S. marcescens can rely on citrate as a carbon source. In identifying the organism, one may also perform a methyl red test, which determines if a microorganism performs mixed-acid fermentation. S. marcescens results in a negative test. Another determination of S. marcescens is its capability to produce lactic acid via oxidative and fermentative metabolism. Therefore, it is said that S. marcescens is lactic acid O/F+.
Test | Result |
---|---|
Gram stain | - |
Oxidase | - |
Indole production | - |
Methyl Red | >70% - |
Voges-Proskaeur | + |
Citrate (Simmons) | + |
Hydrogen sulfide production | - |
Urea hydrolysis | >70% - |
Phenylalanine deaminase | - |
Lysine decarboxylase | + |
Motility | + |
Gelatin hydrolysis, 22 C | + |
Acid from lactose | - |
Acid from glucose | + |
Acid from maltose | + |
Acid from mannitol | + |
Acid from sucrose | + |
Nitrate reduction | + (to nitrite) |
Deoxyribonuclease, 25 C | + |
Lipase | + |
Pigment | some biovars produce red |
Catalase production (24h) | + |
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